Simplecast 2.5.3 full upgrade#

Blinding Cloud range increased from 10 to 11.

Swoop: Orders the Locust to land at the targeted location, allowing it to attack.

Flying Locusts can use Swoop to land and attack

Allows Locusts spawned by Swarm Hosts to fly.

Cost is 200 Minerals, 200 Vespene, 160 seconds.

New upgrade: Flying Locusts - Allows Locusts spawned by Swarm Hosts to fly.

Simplecast 2.5.3 full upgrade#

Enduring Locusts РІР‚вЂњ This upgrade has been removed.

Locust duration increased from 15 seconds (+10 seconds with Enduring Locusts) to 25 seconds.

Casting Spawn Locusts no longer requires a Swarm Host to be burrowed.

Spawn Locusts cooldown increased from 25 seconds to 60 seconds.

Auto-cast removed, Spawn Locusts must now be manually cast.

Spawn Locusts РІР‚вЂњ This ability has been reworked.

Swarm Hosts no longer collide with Locusts.

Swarm Hosts now require the Burrow research in order to burrow.

Movement speed increased from 2.25 to 2.95.

Cost modified from 200 Minerals and 100 Vespene to 100 Minerals and 200 Vespene.

RNA is represented as sticks and the phosphorus atoms are shown as yellow spheres.On April 9, Blizzard released a Balance Update. ( J) Superimposed snapshot of unmodified (blue) and modified siRNA 54 (cyan) AS strands (sense strand is not shown for clarity), illustrating the deviations in the backbone conformation and suboptimal intra-strand base stacking.

The modified linkage is highlighted using an arrow. The black dashed lines indicate the WC H-bond between the bases. Base stacking of the successive base pair steps in the ( H) unmodified and ( I) modified siRNA duplex. Base pairing of the ( F) unmodified and ( G) modified siRNA duplex. (FРІР‚вЂњI) MD snapshot depicting the base pair and base stacking architecture of g4РІР‚вЂњg5 step in unmodified and modified siRNA 54. Base pair step parameters (buckle ( C), slide ( D)Р’В and rise ( E)) of the unmodified and modified siRNA duplexes from the averaged structure obtained from the last 50 ns of the 250 ns MD simulations.

Sugar conformation of nucleotides in unmodified (black bars) versus 2РІР‚Р†-5РІР‚Р† modified (red bars) siRNAs #49 ( A) and #54 ( B). Published by Oxford University Press on behalf of Nucleic Acids Research. Together, these results demonstrate that 2'-5'/3'-5'-modified siRNAs, when properly designed, can offer an efficient new class of siRNAs with diminished immune-stimulatory responses. Importantly, 2'-5'-linkages had the added benefit of abrogating immune-stimulatory activity of siRNAs. Furthermore, our studies indicated that the presence of a single 5'p-rN1-(2'-5')-N2 unit in the antisense strand does not alter the 'clover leaf' bend and sugar puckers that are critical for anchoring the 5'-phosphate to Ago 2 MID domain. Modelling and molecular dynamics simulations were fully consistent with these findings. Extensive modification of the antisense strand minimally affected 5'-phosphorylation of the siRNA by kinases, however, it negatively affected siRNA loading into human AGO2. Screening of these siRNAs against their corresponding mRNA targets showed that 2'-5' linkages were well tolerated in the sense strand, but only at a few positions in the antisense strand. We report on the synthesis of siRNAs containing both 2'-5'- and 3'-5'-internucleotide linkages and their effects on siRNA structure, function, and interaction with RNAi proteins.